A novel SPE-UHPLC-DAD method for the determination of fumagillin produced by Aspergillus fumigatus in cell culture media

Fumagillin is a biomolecule produced by Aspergillus fumigatus that gaining relevance due to its connection with invasive aspergillosis. The determination of this molecule might help understand the propagation disease and study use as potential biomarker. In spite interest fumagillin in microbiological research, no quantitative method has been developed so far for cell culture media. work, first validated analysis RPMI-1640 presented. sample treatment consists mixed-mode anion exchange Solid Phase Extraction effectively removes interferences offered recovery 83 ± 7%. was carried out Ultra High Performance Liquid Chromatography coupled Diode Array Detection at 336 nm. fulfilled validation criteria established EMA FDA guidelines bioanalysis (selectivity, carry over, linearity, accuracy, precision, dilution integrity stability) offers limit quantitation (25 μg·L−1) suitable intended use. Indeed, satisfactorily applied quantification three strains different toxin production capacity.